Pair Name	Gossypol, Idarubicin
Disease Info	[ICD-11: 2A60.Z]	Acute myeloid leukemia		Investigative
Biological Phenomena	Induction-->DNA damage
Gene Regulation	Down-regulation	Phosphorylation	ATM	hsa472
	Down-regulation	Phosphorylation	ATR	hsa545
	Down-regulation	Expression	BCL2	hsa596
	Down-regulation	Expression	BCL-xL	hsa598
	Up-regulation	Cleavage	CASP3	hsa836
	Down-regulation	Phosphorylation	CHEK1	hsa1111
	Down-regulation	Phosphorylation	CHEK2	hsa11200
	Down-regulation	Expression	MCL1	hsa4170
	Up-regulation	Cleavage	PARP1	hsa142
	Down-regulation	Expression	XIAP	hsa331
In Vivo Model	16 male NOD-Prkdc−/−IL2rg−/- mice (NPI, IDMO ltd., Beijing, China) were intravenously injected with 2×10⁶ splenic cells of humanized xenograft mice generated from an AML patient carrying FLT3-ITD mutation via tail vein after irradiation (1 Gy).
Result	These findings suggest that combinatorial therapy with AT-101 and IDA selectively eliminates leukemia stem-like cells both in vitro and in vivo, representing a potent and alternative salvage therapy for the treatment of relapsed and refractory patients with AML.